ELISA assays could not be performed in the field; thus, for screening purposes, definitions were as follows: anemia, Hb <11.5 g/dl; IDE, ZnPP/H >43 μmol/mol (42–44). In the subsequent analyses and for reporting, definitions were as follows: ID, PF <30 μg/liter or sTfR >8.3 mg/liter, in combination with Hb <11.5 g/dl IDA, inflammation/infection CRP >5 mg/liter, and/or AGP >1 g/liter. Vitamin A deficiency was defined with an RBP concentration of <0.7 μM. The amount of absorbed isotopic label (=FAFe) in the blood was assessed by a shift of the isotopic iron ratio in RBCs to calculate total isotopic label using the participant’s blood volume (45) and assuming 80% incorporation of the absorbed iron into RBCs (46). The calculation was conducted using the principles of isotopic dilution and considering that isotopic labels are not monoisotopic (23). We corrected for previously incorporated 54Fe, 57Fe, or 58Fe by using the isotopic ratio value 12 to 16 days after a test meal administration as a new baseline value for the subsequent test meal administration (23).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.