Hb (HemoCue AB, Ängelholm, Sweden) and ZnPP/H (Hematofluorometer 206d, AVIV Biomedical Inc., USA) were measured on blood collection days. Hepcidin [DRG Hepcidin 25 (bioactive) HS, DRG Instruments, Germany] was measured in plasma samples. PF, sTfR, BIS, CRP, AGP, and RBP were analyzed (VitMin Lab, Freiburg, Germany) by sandwich enzyme-linked immunosorbent assay (ELISA), as described elsewhere (41). Detection of malarial parasites in blood smears was done using the Giemsa staining method, and results were supported by rapid malaria diagnostic test kits (SD Bioline). The analysis of blood iron isotopic composition was performed as previously described (23).

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