For the human iron absorption study, six different batches of rice grains were prepared from hot extrusion (~90°C) using stable iron isotopes (54FePP, 57FePP, or 58FePP), as described earlier (17). Batches that were only used for assessment of in vitro iron solubility were produced similarly using nonlabeled iron. The composition of the different batches can be found in table S1. Labeled 54FePP, 57FePP, and 58FePP were prepared in separate batches by Dr. Paul Lohmann GmbH from elemental isotopically enriched iron (Chemgas, Boulogne, France) using a scaled-down process as used for the synthesis of the commercial FePP compound. The batches were then mixed with commercial FePP (Dr. Paul Lohmann GmbH), resulting in an isotopic enrichment of 47.2% 54FePP, 19.8% 57FePP, and 8.6% 58FePP, respectively.

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