HUVECs were plated on coverslips in six-well plates and transfected with si-CCM1 or si-CCM2 for 24 hours. Cells were treated with ponatinib (1 μM) for a further 24 hours before fixation and immunocytochemistry staining with p-MLC2 antibody (Cell Signaling Technology). Images were taken with a Zeiss LSM800 confocal microscope.

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