To quantify colony statistics, Mathematica was used to extract data from the colony images. The following procedure was used (fig. S3): First, background pixels were set to black. Second, the center of mass for the colored pixels was located along with the edge of the colony. Third, each remaining colored pixel was converted in a binary fashion to green or red, depending on the dominant RGB (red, green, blue) value. Connected clusters of red and green pixels were determined for radial cross sections of the colony. Fourth, numbers of red and green pixels in a given cross section were used to determine the statistics of the abundance of each species.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.