Range expansion experiments of bidirectional competing consortia on solid agar
This protocol is extracted from research article:
Spatial interference scale as a determinant of microbial range expansion
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau0695

Single strains of bSRI+ and bLRI+ were grown in LB/Cm/arabinose medium with shaking at 225 rpm and 37°C for 16 hours. Then, the cultures were inoculated at 1:10 dilution into fresh LB/Cm/arabinose medium at 37°C for 5 hours. The OD600 of the two competitor cultures was adjusted to different levels, from 100 to 0.1, using centrifugation or dilution with fresh LB/Cm/arabinose medium. The two cultures were then mixed at different ratios from 10:1 to 1:10. One microliter of aliquot was immediately dropped onto an agar plate (15 ml of LB/Cm/arabinose with 1.5% agar in a 90-mm plate). Droplets were allowed to dry at room temperature for 1 hour, and then, the plates were incubated at 37°C for 80 hours. Control experiments that use the combinations of LRI−/LRI−, bSRI+/LRI−, and LRI−/LRI+ were also investigated. Images were acquired by using a Zeiss Axio Zoom.V16 microscope with an AxioCam HRm camera.

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