Competition assays in liquid culture
This protocol is extracted from research article:
Spatial interference scale as a determinant of microbial range expansion
Sci Adv, Nov 21, 2018; DOI: 10.1126/sciadv.aau0695

Single-strain cultures of SRI+, SRI−, SRI+(kan), SRI−(kan), LRI+, LRI−, LRI+(kan), and LRI−(kan) were grown overnight (16 hours) in LB/Cm/arabinose medium and then subinoculated into fresh media at 1:100 dilution and grown to an OD600 of 0.35. Equal volume of two cultures were mixed in the following combinations: SRI+/SRI−(kan), SRI+/SRI+(kan), SRI−/SRI−(kan), LRI+/LRI−(kan), LRI+/LRI+(kan), and LRI−/LRI−(kan). The well-mixed cultures were further grown at 37°C with shaking at 225 rpm for another 4 hours. Samples were taken from the mixed culture hourly, and colony-forming units of Kan-resistant cells were determined through serial dilution and plate counting with Kan selection.

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