Strains used in this study are all listed in table S1. E. coli 10-beta (New England BioLabs) was used in all cloning steps. E. coli EPI300 (Epicentre) and its derivatives were used to perform both single-cell and droplet competition experiments throughout the study. All E. coli strains were grown in LB medium at 37°C, unless indicated otherwise. If needed, then antibiotics were added using the following final concentrations: chloramphenicol (Cm; 25 μg ml−1) and kanamycin (Kan; 50 μg ml−1).

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