Primary cardiomyocytes were infected with adenovirus expressing GFP alone, β5i, siRNA-control, or siRNA-β5i for 24 hours. Protein lysates were prepared at indicated time points after the addition of cycloheximide (10 μM) or both cycloheximide (10 μM) and MG132 (10 μM). The levels of endogenous ATG5 protein were detected by Western blot analysis with anti-ATG5, quantified, and normalized to GAPDH.

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