The full-length clone DNA of human proteasome (prosome, macropain) subunit β type 8 (large multifunctional peptidase 7) with pCMV3-N-FLAG–expressing plasmid was purchased from Sino Biological Inc. (Beijing, China). The plasmids pEGFP-CMV-flag-GGGS linker-human β5i-GGGS linker-EGFP mutants (1 to 68) (69 to 276) were constructed by Peking Syngenetech Co. Ltd. (China). The plasmids pCMV-myc-ATG5 (human) (1 to 275) and mutant (79 to 275) were constructed by Peking Syngenetech Co. Ltd. (China).

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