The full-length clone DNA of human proteasome (prosome, macropain) subunit β type 8 (large multifunctional peptidase 7) with pCMV3-N-FLAG–expressing plasmid was purchased from Sino Biological Inc. (Beijing, China). The plasmids pEGFP-CMV-flag-GGGS linker-human β5i-GGGS linker-EGFP mutants (1 to 68) (69 to 276) were constructed by Peking Syngenetech Co. Ltd. (China). The plasmids pCMV-myc-ATG5 (human) (1 to 275) and mutant (79 to 275) were constructed by Peking Syngenetech Co. Ltd. (China).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.