H. lacustris NIES-4141 was provided by the Microbial Culture Collection at the NIES. The autotrophic medium AF-6 was used for culture (working volume, 20 ml). Cells were grown in culture flasks under 14-hour light/10-hour dark cycle illumination (120 to 140 μmol m−2 s−1) at 25°C. After a 5-day preculture in normal AF-6 medium, the cells were transferred to nitrogen-deficient AF-6 medium (without NH4NO3 and NaNO3) at a cell density of 1 × 105 cells/ml to induce the production of astaxanthin. The series of measurements were performed 0 to 5 days after the replacement of the culture medium.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.