The FT-CARS spectral acquisition was synchronized with recording of the bright-field images with the high-speed camera. The acquisition was manually triggered on the control software of the high-speed camera, whose ready port was connected to the external trigger port of the digitizer. The duration of the signal acquisition was limited by the memory size of the digitizer, which is 2 gigasamples with 14-bit resolution in our case. At our sampling rate (100 megasamples/s), 20-s acquisition is possible. Continuous limitless samplings can be performed by using a field-programmable gate array to perform real-time signal acquisition and digital signal processing. Discrete spectral acquisition can also be conducted by using the forward-scattered light as a trigger.

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