Bright-field images of flowing cells were acquired simultaneously with their FT-CARS spectra (Fig. 2, A and B). A white light-emitting diode (LED) with a light guide (OptoSigma, SLA100A, MSL-500 L) was used as a light source. Light from the LED was collinearly directed to the cells using a short-pass filter (FESH0650, Thorlabs) with the laser pulses. The transmitted light was reflected by the long-pass filter used for spectral shaping of the laser pulses and focused onto a high-speed camera (Phantom Miro Ex4, Vision research). Bright-field images of the flowing beads and cells were acquired at a frame rate of 10,000 frames/s.

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