Cytoplasmic and nuclear RNAs were purified using the Cytoplasmic and Nuclear RNA Purification Kit (Norgen) according to the manufacturer’s instructions. Briefly, 3 × 106 MDA-MB-231 LM2 cells were harvested and incubated with lysis solution for 5 min. After centrifugation, cytoplasmic RNA was isolated from the supernatants, and nuclear RNA was isolated from the pellets.

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