5′-RACE and 3′-RACE analyses were performed using the SMARTer RACE cDNA Amplification Kit (Clontech Laboratories) according to the manufacturer’s instructions. Briefly, total RNA from MDA-MB-231 cells was extracted with an RNeasy Mini Kit (QIAGEN), and gDNA was removed by on-column deoxyribonuclease I [ribonuclease (RNase) free; New England Biolabs] digestion. After a poly(A) tail was added using poly(A) polymerase (TAKARA), 5′-RACE and 3′-RACE products were amplified with their respective lncRNA-specific primers and cloned into the pGEM-T Easy Vector (Promega) for sequencing, and the spliced full-length lncRNAs were obtained using a new pair of primers. The poly(A) tail detection assay was performed using total RNA from MDA-MB-231 cells, which were either treated or not treated with poly(A) polymerase (TAKARA). The relative abundance of TROJAN in poly(A) polymerase–treated or untreated RNA was determined by qPCR. Glyceraldehyde-3-phosphate dehydrogenase [containing poly(A)] and U6 [without poly(A)] were used as reference genes.

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