Two gRNA sequences spanning the TROJAN genomic locus were designed with the CRISPR Design Tool (https://zlab.bio/guide-design-resources). A one-step cloning method was used to generate two single gRNAs in LentiCRISPRv2 as previously described (40), with the exception that one of the U6 promoters was replaced with an H1 promoter to avoid potential homologous recombination. The H1 promoter sequence was amplified from pUC-H1-gRNA (Addgene plasmid 61089). The TROJAN deletion efficiency was measured by PCR. The following gRNA was used for ZMYND8 knockout: 5′-GTGATGTGTCCTGCGGCGAG-3′. Single clones were established by dilution cloning.

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