Total RNA was isolated from patient specimens or cells using the RNeasy Plus Mini Kit (QIAGEN) following the manufacturer’s protocol. First-strand cDNA synthesis from total RNA was performed using the PrimeScript RT Reagent Kit with gDNA (genomic DNA) Eraser (TAKARA). Real-time qPCR was performed using SYBR Premix Ex Taq (TAKARA) on an ABI 7900HT Fast Real-Time PCR system (Applied Biosystems). The results were analyzed with SDS v2.1 software and the 2−ΔΔCt method.

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