Single-cell velocities
This protocol is extracted from research article:
Swimming bacteria power microspin cycles
Sci Adv, Dec 19, 2018; DOI: 10.1126/sciadv.aau0125

E. coli was grown for 5 to 7 hours, centrifuged, and resuspended in TB at low volume fraction before being plated in tunnel slides constructed using two layers of double-sided tape as spacers between slide and coverslip. Ten-second DIC movies were taken as described above. Individual cells were identified in our images using nonnegative mixed-norm preconditioning (30) to threshold the images. The resulting binary images were then used to track the center of mass for individual cells, and the instantaneous velocity was computed as the difference in center of mass position between successive frames. The instantaneous velocity was then averaged for each cell individually.

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