Bacterial density measurements
This protocol is extracted from research article:
Swimming bacteria power microspin cycles
Sci Adv, Dec 19, 2018; DOI: 10.1126/sciadv.aau0125

Cultures were stained with 4 μM FM 4-64 (Life Technologies) for 20 min and were then centrifuged and pelleted as above. After removal of the supernatant, the bacteria were resuspended in 25 μl of TB. Droplets were created by pipetting and mixing the culture with 100 μl of mineral oil with DiPhyPC and were sandwiched between silane-coated coverslips. For each droplet, a 10-s DIC movie was captured, immediately followed by a 10-s epifluorescence movie. Each of these movies was acquired at a rate of 16 fps with 2 × 2 binning.

Average fluorescence intensity was determined within droplets using the ImageJ Z-project tool to average the intensity over a specific length of time (typically 1 s). To confirm asymmetry in the intensity distribution, the plot profile tool was used to measure the intensity across two perpendicular cuts through each averaged fluorescent image.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.