NMR measurements for water in different annealed PVA samples
This protocol is extracted from research article:
Heterogeneous ice nucleation correlates with bulk-like interfacial water
Sci Adv, Apr 12, 2019; DOI: 10.1126/sciadv.aat9825

The proton spin-spin relaxation time (T2) measurements of water in different annealed PVA films were carried out on a Bruker AV600 NMR spectrometer using the Carr-Purcell-Meiboom-Gill pulse sequence. A spacing of 1.2 ms between the 90° and 180° pulse was used, and a relaxation delay of five times of the spin-lattice relaxation time between consecutive scans was necessary to ensure full recovery of the magnetization between acquisitions. The annealed PVA films were rolled and stuffed in the NMR test tube, which was filled with H2O and D2O (volume ratio of 1:1). All the T2 relaxation behaviors for the water proton were fitted with a biexponential decay curve. T2 of water was also measured as a function of temperature in a wide range from −10.0° to 20.0°C.

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