We used the utility scripts provided by Trinity to automate the alignment of the reads from each tissue to the transcriptome using Bowtie2 (version 2.2.0, https://github.com/BenLangmead/bowtie2). Subsequently, the gene expression level based on the aligned reads was estimated using RSEM (version 1.2.31, https://github.com/deweylab/RSEM). Normalization across samples was applied using the trimmed means of M values, and differential expression analysis was performed using the DESeq2 package (https://bioconductor.org/packages/release/bioc/html/DESeq2.html).

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