Before transcriptome assembly, adapters and low-quality reads (minimum read length, 35 bp) were removed using Cutadapt ( Because the available P. parnellii genome (4) is highly fragmented, we used the Trinity (version 2.0.6) de novo assembly package ( to assemble the P. parnellii transcriptome. Highly similar transcripts were collapsed using CD-Hit ( Annotation of the transcriptome was carried out using Blast+ against human complementary DNA sequences from Ensembl (version 86). We kept the best Blast hit with the lowest E value and highest percent identity, which associates each transcript to a human Ensembl gene.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.