Before transcriptome assembly, adapters and low-quality reads (minimum read length, 35 bp) were removed using Cutadapt (https://cutadapt.readthedocs.io/en/stable/). Because the available P. parnellii genome (4) is highly fragmented, we used the Trinity (version 2.0.6) de novo assembly package (https://github.com/trinityrnaseq/trinityrnaseq) to assemble the P. parnellii transcriptome. Highly similar transcripts were collapsed using CD-Hit (http://weizhongli-lab.org/cd-hit/). Annotation of the transcriptome was carried out using Blast+ against human complementary DNA sequences from Ensembl (version 86). We kept the best Blast hit with the lowest E value and highest percent identity, which associates each transcript to a human Ensembl gene.

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