Construction of physical models
This protocol is extracted from research article:
Manta rays feed using ricochet separation, a novel nonclogging filtration mechanism
Sci Adv, Sep 26, 2018; DOI: 10.1126/sciadv.aat9533

Mobulid filters are composed of repeating filter lobes connected by a central cartilaginous raphe [see Paig-Tran et al. (18) for full description]. Computer models of the filtering apparatus of M. birostris were constructed from the measured morphology (Autodesk software 123D Design), and then, physical models were 3D printed. For the filtration efficiency experiments, multiple rows of filter lobes were positioned together so that they could be inserted into the bottom surface of the flow tank (fig. S1A). Models were printed at 1× scale [32-μm layer thickness, 10 filter rows in lateral direction, and 13 filter lobes in streamwise direction; ProJet 3510SD (3D Systems)]. For the dye visualization experiments, models consisted of a single row of filter lobes at 4× scale [100-μm layer thickness, one filter row in lateral direction, and 13 filter lobes in streamwise direction; ProJet 460 (3D Systems)]. For particle tracking experiments, models were also a single row of filter lobes but were printed at 1× scale [50-μm layer thickness, one filter row in the lateral direction, and 13 filter lobes in streamwise direction; Form 2 printer (Formlabs)].

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