Specimen collection and examination
This protocol is extracted from research article:
Manta rays feed using ricochet separation, a novel nonclogging filtration mechanism
Sci Adv, Sep 26, 2018; DOI: 10.1126/sciadv.aat9533

The morphology of the M. birostris filtering apparatus was measured from two specimens (one provided by the Smithsonian Museum of Natural History and one donated by R. Rubin and maintained by A. Summers). Calipers were used to measure the dimensions of the filter lobes (fig. S2B). The morphology of M. tarapacana was measured by performing a μCT scan of a specimen provided by the Scripps Institution of Oceanography. M. tarapacana rakers were scanned using high-resolution μCT imaging (Bruker SkyScan 1272) scanned at 60 kV, with a resolution of 38.9 μm at the University of Washington Friday Harbor Laboratories. μCT scans were then reconstructed from 8-bit tagged image file format stacks as 3D images using Amira software (version 6.4). The μCT model was then used to measure the dimensions of the filter lobes (fig. S4).

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