This methodology was taken from our previous study (12). All animals were sacrificed 3 weeks after MI induction, after the echocardiography study. Hearts were collected and cryopreserved in OCT compound. Heart sections (10 μm thick) were cut from the apex to the height of the ligation. Each section was cut at 100-μm intervals. Masson’s trichrome [HT15 Trichrome Staining (Masson) Kit, Sigma-Aldrich] staining was performed according to the manufacturer’s specifications. A PathScan Enabler IV slide scanner (Advanced Imaging Concepts, Princeton, NJ) was used to acquire images of each stained section. These were used to assess morphometric parameters (i.e., infarct thickness and viable myocardium), which were quantified with NIH ImageJ software.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.