All animal work was compliant with the Institutional Animal Care and Use Committee at North Carolina State University. Briefly, rats were anesthetized with ketamine. Immediately after MI induction (49), animals were randomized to receive one of the following two treatments: (i) MI + No–MN-CSC group: a PVA patch without MNs containing 1 × 106 CSCs was placed onto the infarcted heart; (ii) MI + MN-CSC group: a MN-CSC patch containing 1 × 106 CSCs was placed onto the infarcted heart. Serum ALT, AST, creatinine, and BUN levels were measured 21 days after transplantation. Normal range for rats is as follows: ALT, 10 to 40 IU/liter; AST, 50 to 150 IU/liter (50); creatinine, 0.1 to 0.55 mg/dl; BUN, 3.56 to 25.43 mg/dl (51).

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.