NRCMs were derived from Sprague-Dawley rats as previous described (48). We cultured NRCMs in a four-well chamber. A MN or MN-CSC patch was placed on the surface of NRCMs. A solitary NRCM culture was included as a control. A LIVE/DEAD Viability/Cytotoxicity kit was used to determine the cell viability of NRCMs at day 3. The morphology of the cells was characterized using National Institutes of Health (NIH) ImageJ software. Cell proliferation was evaluated by the percentage of α-SA/Ki67-positive cardiomyocytes by immunocytochemistry staining.

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