This methodology was taken from our previous study (47). Rat CSCs (1 × 105) or MN-CSC containing 1 × 105 rat CSCs were cultured in 1 ml of FBS-free media in a 24-well plate. Conditioned media were collected from the plates on days 1, 3, and 7 to study the cells’ release of growth factors. The concentrations of various growth factors in the media were measured by ELISA kits (R&D Systems, Minneapolis, MN; B-Bridge International, Cupertino, CA) as per the manufacturer’s instructions. The growth factors assayed were HGF, VEGF, and IGF-1.

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