Male Kunming mice and female Balb/c mice injected with MoS2 nanosheets or DOX/PEG-MoS2 nanosheets were sacrificed by cervical dislocation after a specified time period, and organs studied in this paper were harvested within 10 min. Harvest organs were snap-frozen in liquid nitrogen and then stored in the fridge at −20°C. Before LDI MSI, the frozen tissues were cryosectioned at 10-μm-thick sections using a Leica CM1950 cryostat [Leica Biosystems (Nussloch, Germany)], and then, tissue sections were thaw-mounted onto the conductive side of an indium tin oxide (ITO)–coated glass slide [Bruker Daltonics (Bremen, Germany)]. For tissues that need H&E staining, tissues adjacent to the section for LDI MSI were mounted onto adhesion microscope slides. The ITO-coated glass slides with tissues were placed into a vacuum desiccator for about 30 min to completely dry the tissue.

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