DOX was loaded on the LA-PEG–modified MoS2 nanosheets by mixing the PEG-MoS2 nanosheets (0.2 mg/ml) with saturated DOX solution [in 10 mM PBS (phosphate-buffered saline) buffer (pH 8.0)] and stirring at room temperature for 24 hours. After loading, the excess free DOX was removed by centrifugal filtration and washed with PBS buffer several times. The amount of loading DOX was determined by measuring the characteristic peak intensity UV-vis adsorption spectra of PEG-MoS2 nanosheets and DOX/PEG-MoS2 nanosheets. The concentration of PEG-MoS2 nanosheets was determined by adsorption at 808 nm, and the concentration of DOX was determined by adsorption at 490 nm after subtracting the contribution by PEG-MoS2 nanosheets.

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