To test for correlation between the frequency of best hearing sensitivity and the functional parameter 1/α of prestin, we combined related data from previous studies (19, 28) with those of our study. The frequencies of best hearing were from published audiograms, and if no audiogram was available, then the values were inferred on the basis of published call frequencies with maximum energy for the species (28). The correlation between the NLC parameters and mammalian hearing frequencies, as well as statistical tests, were carried out with program R. To exclude the influence of phylogeny on the correlation analysis, we conducted an independent contrasts test (29) based on a species tree with estimated divergence times (28, 50).

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