Reconstruction of prestin sequences for ancestral whales

To more reliably infer the ancestral prestin sequences in whales, we added another 10 whale species with intact coding sequences of prestin in GenBank (www.ncbi.nlm.nih.gov/genbank/). The final data set included 11 echolocating toothed whales and 3 nonecholocating baleen whales, covering the main lineages of living whales (Fig. 3A and table S7). After alignment, we inferred the prestin sequences of ancestral whales using the same approaches described above. A statistical test and its improved version (31, 32) were used to calculate the probability that the number of observed convergent sites exceeded that expected by chance.

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