SecA was diluted to 20 nM and incubated for 15 min in one of two solutions: (i) no substrate (apo) conditions [10 mM Hepes (pH 7.6), 100 mM KAc, and 5 mM MgAc2] or (ii) saturating nucleotide (ATP, ADP, or ADP-AlF3) conditions [10 mM Hepes (pH 7.6), 100 mM KAc, 5 mM MgAc2, and 100 μM nucleotide]. One hundred fifty microliters of this solution was then deposited onto a freshly cleaved mica surface. After 10-min incubation on mica, the sample was rinsed 10 times with 100 μl of the appropriate solution.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.