SecA-WT was purified following standard protocols described in reference (7). The SecAΔPBD mutant (amino acids 231 to 369 deleted) is SecAC4 [a SecA variant that lacks zinc because the coordinating cysteines have been replaced by serine (26)] engineered to remove the protein-binding domain. The construct was made commercially (Mutagenex Inc.), and the protein was purified as described (7).

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