SecA-WT was purified following standard protocols described in reference (7). The SecAΔPBD mutant (amino acids 231 to 369 deleted) is SecAC4 [a SecA variant that lacks zinc because the coordinating cysteines have been replaced by serine (26)] engineered to remove the protein-binding domain. The construct was made commercially (Mutagenex Inc.), and the protein was purified as described (7).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.