The procedure was done following the steps outlined by Jang et al. (44). Briefly, for the functionalization of silica particles, 0.23 g of silica pellet (as prepared above) was redispersed in 40 ml of fresh ethanol in a sonication bath. Under stirring with a Teflon-coated magnetic bar, 4 ml of ammonia solution (0.727 M in ethanol) was added. APTES solution (170 μl; 0.011 M in ethanol) was then slowly added dropwise to the silica dispersion. After 15 hours, the resultant solution was washed with ethanol five times by centrifugation to remove the unreacted APTES molecules.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.