All glass surfaces in contact with the emulsions, from the vials to the slides to the transfer pipettes (Fisher Scientific), were all treated with PDADMAC before use, as described in the “Materials” section. Samples were always made with fresh surfactant stock solutions to minimize the effects of hydrolysis and of carbon dioxide absorption and were measured to have a pH range of 7 to 7.5. When titrating components into the solution, nanoparticles were diluted first, then the pH was adjusted by additions of either HCl or NaOH. This was vortexed before the surfactant was added. The nanoparticle solution was then bath sonicated for 30 min before the cholesteric liquid crystal was introduced. Approximately 10 μl of the liquid crystal was pipetted to 1 ml of the nanoparticle solution. Liquid crystal in water emulsions was then created by simple shaking. Samples were viewed under the confocal microscope in a covered, hydrated container to minimize evaporation.

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