Fluorescent lipid patterning on a thin cholesteric film
This protocol is extracted from research article:
Shaping nanoparticle fingerprints at the interface of cholesteric droplets
Sci Adv, Oct 12, 2018; DOI: 10.1126/sciadv.aat8597

With DLPC (Avanti Polar Lipids), labeled with 1 mol % TR-DHPE, as the surfactant, the water phase is a tris-buffered saline solution (10 mM tris, Fisher Scientific; 0.1 M NaCl, adjusted to pH 8.9 with HCl, Fisher Scientific) with a dispersion of vesicles 50 nm in diameter, following the procedure of previous work (14). A similar procedure from this work was also used for creating a thin cholesteric film in a copper transmission electron microscope grid on a cover glass treated with octadecyltrimethoxysilane (Sigma-Aldrich).

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