Sample 2 of the Senegal nest wall, which was prepared for x-ray microtomography imaging, was mounted on a sample holder. A total volume of 0.16 ml of 23 weight % potassium iodide (KI) (puriss, 99.5%, Sigma-Aldrich, UK) aqueous solution was injected at the top of the sample. The water was doped with KI to enhance the x-ray contrast between air and the aqueous phase (Fig. 6). The sample was then wrapped with a cling film to avoid any evaporation of the aqueous phase during scanning. The tomographic image represents the sample at approximately 2.5 hours. The experiment was conducted at ambient temperature (20°C).

The air phase was segmented with a seeded watershed algorithm based on the grayscale gradient and grayscale intensity of each voxel. An axis connectivity analysis was performed on the isolated air phase using Avizo-9 software to confirm the percolation of the air phase between the two sides of the sample.

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