The molar fraction of aggregated molecules (αagg) at a certain temperature was calculated from the absorption intensity at λ = 470 nm based on Eq. 3, in which Abs(agg) and Abs(mono) are the absorption intensities of fully aggregated (at the lowest temperature) and purely monomeric states (at the highest temperature), respectively, and Abs(T) is the absorption intensity at a given temperature (T)Embedded Image(3)

The plot of αagg versus temperature provides heating curves with either nonsigmoidal (cooperative mechanism) or sigmoidal (isodesmic mechanism) shape, which were fitted using the models proposed by Jonkheijm et al. (30) (for cooperative mechanism) and Martin (31) (for isodesmic mechanism), respectively. The standard values of enthalpy (ΔH°), entropy (ΔS°), and Gibbs free energy (ΔG°) were calculated using the van’t Hoff equation.

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