Dot plots, bar charts, and line charts were generated in GraphPad Prism or Excel. GraphPad Prism and Excel were also used for statistical analyses of these data, including ensuring that data met assumptions of the tests used and comparisons of variance between groups when appropriate. Microsoft Excel was used to perform two-tailed t tests. A three-dimensional scatterplot was generated in R using the rgl package. Flow cytometry data were prepared for display using FlowJo software. Microarray gene expression data were displayed as a heatmap prepared using Genesifter software. Heatmap of MEMA data was generated in R using the standard heatmap function and default parameters.

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