All mouse experiments were performed in accordance to the guidelines issued by the Animal Care and Use Committee at OHSU or the Fred Hutchinson Cancer Center, using approved protocols. Mice were housed in a specific pathogen–free environment under strictly controlled light cycle conditions, fed a standard Rodent Laboratory Chow (#5001 PMI Nutrition International), and provided with water ad libitum. The following strains were used in the described studies: C57BL/6J (JAX #000664), Gt(ROSA)26Sortm(EYFP)Cos/J (R26R-stop-YFP; JAX#006148) (21), Tg(act-EGFP)Y01Osb (Act-GFP; JAX #006567) (20), and B6.129P2-Lyz2tm1(cre)Lfo/J (LysM-Cre; JAX#004781) (52). Mice of both genders were randomized and analyzed at 8 to 10 weeks of age. When possible, controls were littermates housed in the same cage as experimental animals.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.