Cells were stained with a phycoerythrin (PE)–labeled mouse anti-human CXCR4 antibody (BD Biosciences, San Jose, CA), a PE-labeled rat anti-human CCR7 antibody (BioLegend, San Diego, CA), or a mouse anti–human α4β7 integrin antibody (Act-1) (obtained from the NIH AIDS Reagent Program), followed by secondary antibody staining with Alexa Fluor 647–labeled goat anti-mouse antibodies (Invitrogen, Carlsbad, CA). Cells were stained on ice in phosphate-buffered saline (PBS) + 0.1% bovine serum albumin (BSA) for 30 min, washed with cold PBS–0.5% BSA, and then analyzed on FACSCalibur (BD Biosciences, San Jose, CA).

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