One million cells were fixed, permeabilized, washed, and then stained with an anti-human p-cofilin antibody using an intracellular protein staining kit (a gift from Virongy, Manassas, VA) for 60 min at room temperature. Cells were washed twice and stained with Alexa Fluor 488–labeled chicken anti-rabbit antibodies (Invitrogen, Carlsbad, CA). Cells were washed twice and then analyzed on a FACSCalibur (BD Biosciences, San Jose, CA). Multiple donors were used for intracellular p-cofilin staining.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.