Purified CVA10 viruses were diluted in PBS buffer and then absorbed onto 200-mesh carbon-coated copper grids for 1 min. The grids were washed twice with double-distilled water and subsequently negatively stained with 2% phosphotungstic acid (pH 6.4) for 30 s. Specimens were evaluated and imaged with the FEI Tecnai T12 transmission electron microscope at a magnification of 25,000×.

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