Two-photon fluorescence images were obtained using a Zeiss LSM 510 confocal laser scanning microscope (Carl Zeiss MicroImaging, Jena, Germany). Thioflavin S was excited at 750 nm with a mode-locked Ti-sapphire laser (Chameleon, Coherent Inc., Santa Clara, CA), and fluorescence emission was collected in the range of 535 to 590 nm.

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