RD cells were preseeded in 96-well plates and ready for two assays. Each incubation in both assays was performed at 4°C with a duration of 1 hour unless otherwise stated. In the pre-attachment neutralization assay, serially diluted mAb 2G8 was incubated with CVA10 and then added to the cells for one more incubation. In the post-attachment neutralization assay, the cells were first incubated with CVA10 and then washed twice with cold PBS buffer. Different dilutions of mAb 2G8 were subsequently added for one more incubation. The cells were finally washed twice with cold PBS buffer and incubated at 37°C for 4 days. The CPE was observed under the microscope.

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