In prevention experiments, groups of neonatal mice were inoculated intraperitoneally with 100 μl of either antisera or NAb 2G8 (30 μg for each gram of body weight) 12 hours before intraperitoneal inoculation with 100 μl of CVA10 (106 TCID50 per mouse). In treatment experiments, mice were simply inoculated intraperitoneally with NAb 2G8 24 hours after inoculation of CVA10. The mice in the control groups were treated with PBS buffer via the same route. Every group comprised 8 to 10 mice. All mice were monitored daily for clinical illness and death until 20 d.p.i.

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