RD cells were preseeded in 96-well plates with 1 × 104 cells per well. Antisera or mAb 2G8 was serially diluted twofold and incubated with an equal volume of CVA10 (100 TCID50 per well) at 37°C for 1 hour. The mixtures were then added to cells and incubated at 37°C for 5 to 7 days. The cytopathic effect (CPE) was observed with microscopy, and a neutralizing titer was defined as the highest dilution giving >50% neutralization of the well. A neutralizing titer of antisera with values of ≥1:8 was considered as a threshold for positivity.

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