The immunogenicity of the CVA10 virus was evaluated in BALB/c mice. The purified CVA10 mature virions and A-particles were inactivated by heating at 56°C for 30 min. Viruses were diluted in PBS and mixed with an equal volume of aluminum adjuvant. Groups of mice (n = 5) were respectively vaccinated with mature virions, HI mature virions, A-particles, HI A-particles, procapsids, or aluminum adjuvant (control) at weeks 0 and 3. Each mouse was intraperitoneally injected with 0.5 ml of the samples at 2.5 μg per dose. Antisera elicited from all groups were inactivated by incubation at 56°C for 30 min and stored at −20°C. All animal experiments were approved by the Institutional Animal Care and Use Committee and Laboratory Animal Management Ethics Committee at Xiamen University.

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