ThermoFluor assay (42) was performed with an MX3005P RT-PCR instrument (Agilent/Stratagene). The presence of single-stranded RNA was detected with SYTO9 (Invitrogen) as a fluorescent probe. Each 50-μl reaction mixture, containing 1 μg of virus and 5 μM SYTO9 in PBS buffer, was set up in thin-walled PCR plates (Agilent). The fluorescence level was recorded in triplicate at temperatures between 25° and 99°C at 0.5°C intervals.

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